Instrument
Excitation: Quartz tungsten-halogen source lamp, 5 position excitation filter wheel
Detector: Single scanning photomultiplier tube (PMT), 5 position detection filter wheel
Filters: Customizable filter wheel with five (5) user-selecte
Instrument
Excitation: Quartz tungsten-halogen source lamp, 5 position excitation filter wheel
Detector: Single scanning photomultiplier tube (PMT), 5 position detection filter wheel
Filters: Customizable filter wheel with five (5) user-selected filters (excitation and detection)*
Thermal system: Solid state, Peltier-based, 96-well block thermal system
Sample format: Standard 96-well plates, 8-strip tubes, 200?l tubes, optimized for 25?l reactions
Embedded computer collects and saves experiment data during power failure or computer crash
*Choice of filters includes: ALEXA Fluor? 350 (350nm-440nm), FAM™/SYbr? Green I (492nm-516nm), TET (517nm-538nm), HEX™/JOE™/VIC™ (535nm-555nm), Cy3™ (545nm-568nm), TAMRA™ (556nm-580nm), ROX™/Texas Red? (585nm-610nm), and Cy5™ (635nm-665nm).
System Specifications
Linear dynamic range: 10 orders of magnitude, standard curve method
Excitation range: 350 to 750 nm
Emission range: 350 to 700 nm
Temperature uniformity: +/- 0.25 ˚C at 72 ˚C
Amplification rate: standard 40 cycle 2-step QPCR reaction completed in 90 minutes; the same reaction is completed in 60 minutes using FullVelocity™ SYBR? QPCR & QRT-PCR reagents (without dissociation)
Discrimination: distinguish samples with 5,000 and 10,000 template copies with a 99.7% confidence level
Detection down to single-copy equivalent
Dimensions: 13" (33cm) W x 18" (46cm) D x 17"(43 cm) H, Weight: 44 lbs. (20 kg)
Power: 100 to 230 VAC +/- 10%, 50/60 Hz
MxPro™ QPCR Software
Multiplex up to 5 dyes simultaneously , customer-selected filter set
Independent filter wheel alignment feature enables using FRET chemistry applications
Multiplex plate setup does not require layering (no switching required between dye layers)
Perform multiple sub-experiments on a single plate; data available as raw fluorescence data
Each experiment type includes default setup and automatically displays appropriate analysis view
Ability to start a run in one step using experiment type defaults
Monitor real-time amplification plots and data analysis during a PCR run
Flexible analysis options: amplification plots, scatter plots, standard curve charts, fluorescence intensity, final call results, melting curve, annealing range and text report
View all dyes for multiplex experiments on one amplification plot and/or standard curve chart or view individual plots for each individual dye
Melting curve analysis with automatic melting temperature (Tm) calculation
Allelic discrimination by threshold cycle or by endpoint fluorescence values with auto-call feature
Export chart and text report data directly from display screen to Microsoft? Excel, PowerPoint, text and image files
Software can link up to six (6) Mx3005P and Mx300P instruments with one computer
Links
